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The flanking sequences of the 3′ LTR were left unaltered by this strategy. Transfection of these chimeric vectors into 293T cells and harvest of supernafefabtants 48 h later。

The molecular clone of feline foamy virus (pCF-7) and FeFAB cell for FeFAB assay were kind gifts from Dr.fefab Martin Lochelt, German Cancer Research Center, Germany.

HEK293T cells were cotransfected with pCF-BBtr, the empty vector pcDNA, or pcfeA3Z2b-HA, and increasing amounts of plasmids encoding either wt or mutant Bet (Figure。

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